Cell3iMager duos2 Application Note　

Respiratory diseases include a wide range of conditions, including
chronic progressive diseases and acute and severe diseases such as
the recent COVID-19 pneumonia. However, the development of
fundamental therapeutics in this field has been slow, partly because
animal models such as mice used for preclinical drug efficacy evaluation
often do not reflect human disease states. A typical example is
cystic fibrosis (CF), an autosomal recessive genetic disorder caused
by mutations in the cystic fibrosis transmembrane conductance
regulator (CFTR), a chloride ion channel.

In recent years, NK (Natural Killer) cells have been attracting attention in the fields of drug discovery development and cancer research. NK cells are a type of lymphocyte that works in innate immunity and play a major role in the removal of tumor cells and virus-infected cells.
By using Cell3iMager duos / duos2 and Deep Learning Plug-In, you will be able to perform a long-term Killing Assay. Since the imager uses a plate-fixed imaging method, clear imaging is possible even for suspended-cultured NK cells and 3D-cultured cancer cells.

With this study we conclude that Cell3iMager duos imaging technology in conjunction with deep learning is highly suited for delineating various biological processes using sympathetic neurons derived from hiPSC. Additionally, the platform can be used for identifying drug entities that could stimulate or block neuronal outgrowth. The analysis by deep learning accurately detects cells and is highly robust compared to traditional analyses even if there are differences in brightness or in the presence of debris.

Cell3iMager duos / duos2 technology is highly suited for brightfield/fluorescence imaging of 3D cultured organoids. We conclude that the Label-free analysis is highly advantageous and could substitute microscopic method of monitoring of organoid growth processes, as segmentation of each organoid shape is possible using Deep Learning. Similarly, label-free analysis may also substitute ATP assays, toxicity assessments performed by fluorescent staining, and FIS assays.

Isolation of single cell derived clone with Duos superior imaging technology.

Utility of Cell3iMager duos imaging platform for isolation and assessment of single cell derived clone

The cell density that stepwise cell number seeding, was quantyfied by using Cell3iMager duos.

Hypoxia level of 2D/3D-cultured cells was evaluated by using Cell3iMager duos.

Differentiation efficiency of β-NGF-treated PC12 was evaluated by using Cell3iMager duos.

Spheroids were cultured with EZSPHERE® PLATE and analyzed using Deep Learning plug-in

Cell viability in the presence of drug is calculated by using Calcein-AM/PI-double staining.

Viability of anti-cancer drug treated cells is quantified by using Cell3iMager duos.

Multi spheroids cultured in FCeM-medium were quantified by using Cell3iMager duos.

Visualisation and Quantification of spheroid volumes cultured in Cell3iMager duos.

The cell density that stepwise cell number seeding, is quantified by using Cell3iMager duos.

Suspension cultured cells were quantified by using Cell3iMager duos.

Invasiveness of MCF-7 (low malignant tumor) and MDA-MB-231 (high malignant tumor ) cells are compared by using Cell3iMager duos.

Anti-cancer drug screening in TCF4/LEF reporter (GFP) -expressing cells is performed by using Cell3iMager duos.

Spheroids in microwells of Corning®Elplasia® plate made by Corning are scanned and measured by Cell3iMager duos.

Proliferation ratio of the 2D/3D cultured cells is quantified by Cell3iMager duos.

We performed the labeled-free colony formation assay using Cell3iMager duos.

Jarkat cells induced cell death by Anti-Fas antibody were classified by Cell3iMager duos and Deep Learning.