A bright field spheroid counter, allowing reagent-free follow-up and morphological observation
3D Cell scanner
Cell3iMager neo
Quantification of 3D cultured cells in just 90 seconds
- Bright-field, label-free, non-invasive measurement
- Automatic imaging and quantification at a reasonable price
High-throughput imaging
abel-free analysis enables continuous observation
Label-free and non-invasive analysis using bright field, allowing automatic measurement of course and morphology in the same wells, and continuous observation to graph the course of growth and save images.
Affordable and Automated Imaging
The Entry Model to High-Speed Imaging System, specialized for 3D cultured cells, provides the convenience of automated imaging and analysis at an affordable price.
Features
High-throughput imaging
- Rapid quantification of 96 wells in 90 seconds
- High speed imaging and high speed analysis make 3D cultured cell quantification more accurate and efficient.
- In addition, it supports automation, allowing users to build a workflow that suits them, such as scanning first and analysis later.
Imaging system specialized for 3D culture
- All-in-focus images, which are synthesized from images captured at multiple depths of focus, can be used to analyze cells scattered in the thickness direction.
- Integrating information in the thickness direction allows accurate quantification of three-dimensional cultures, such as agar and floating culture systems.
High correlation with ATP assay
- Software and transillumination provide accurate supplementation to spheroids around the wells and analogize the volume value of spheroids.
Time course statistics
- Different culture environments and compound test patterns can be grouped in a well plate.
Selectable scan resolution
- Adjustable scanning accuracy from 200 dpi to 9600 dpi (2.6 μm/pixel) to match the sample and analysis speed(7 levels: 200, 300, 600, 1200, 2400, 4800, 9600 dpi)
Software for 3D Culture
- Multi-focus scanning in the Z-axis ensures that no cells are missed, even in 3D cultures such as gel-embedded cultures.
- Supports a variety of analytical screening such as kinetic analysis and temporal analysis.
- More than 30 parameters are employed to accurately separate the sample signal from the artificial signal.
- This enables accurate extraction and viability determination of cultured cells.
- Supports quantification of single spheroids and multiple spheroids.
Application note
3D Cell Culture in Gravity TRAP™
Spheroids formed by the method of hanging drop were moved to Gravity TRAPTM ULA plate, and quantified by Cell3iMager neo.
3D Cell Culture by Hanging Drop
Spheroids in the droplets being aggregated by the method of hanging drop were quantified by Cell3iMager neo
Application note Vol.1
Development of spheroid-based anticancer drug screening method using high-speed cell culture scanner